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Kite Tenjo's laboratory is a location where Kite Tenjo and Orbital 7 carry out various experiments to locate the "Numbers" as well as various other interests. It is located near the Heartland Pier in the Yu-Gi-Oh! ZEXAL anime.
Orbital 7 found out that Yuma Tsukumo's Emperor's Key is related to the "Number" cards here, thus visited the Heartland Middleschool under Kite's orders to retrieve it, otherwise he would be turned to scrap.
After Kite defeated Reginald Kastle above the school, he flew away with the claimed key and began to study it here. He used Orbital 7's Baria Crystal energy in order to force the key to produce a portal which he could enter. He then entered to Duel Astral and claim the "Numbers" which he holds.
In order to get Astral back, Yuma and his friends ask Mr. Kay to send one of his famous Crashbug Viruses throughout the city in order to locate the key. When one of the virus particles reports the location, Yuma and co run to it. When they get there, the defenses of the facility are too strong but thanks to Mr. Kay's virus, the doors open.
When Yuma manages to get to the control room, he is faced with more of the buildings complex defences to which when he presses the buttons on, an image of Kite and Hart appears, shocking Yuma. When Orbital 7 sees that Yuma is outside the control room, he tries to attack him but fails as his drills get stuck in the wall. Yuma then runs into the control room and enters the portal that Kite and Orbital 7 created.
The building later exploded, which Yuma's friends think killed him as they didn't see him exit, but he managed to escape it by hiding in the Emperor's Key until the flames had died down. During the opening ceremonies of the World Duel Carnival, Mr. Heartland questioned Kite on the mysterious explosion which occurred here which Kite replies that there was a malfunction with some of the machinery (in the dub, Kite lied to Mr Heartland that he didn't know anything about the incident). Later in Yu-Gi-Oh! ZEXAL II, this laboratory was rebuilt at Heartland Tower as a place for Kite and Orbital 7 to research about the Barians.